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Suppressed TGF‐β1 expression is correlated with up‐regulation of matrix metalloproteinase‐13 in keloid regression after flashlamp pulsed‐dye laser treatment
Author(s) -
Kuo YurRen,
Wu WenShan,
Jeng SengFeng,
Wang FengSheng,
Huang HuiChen,
Lin ChaZon,
Yang Kuender D.
Publication year - 2005
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20104
Subject(s) - keloid , immunohistochemistry , extracellular matrix , matrix metalloproteinase , western blot , staining , transforming growth factor , pathology , medicine , chemistry , biochemistry , gene
Background and Objectives Flashlamp pulsed‐dye lasers (PDLs) has shown effectiveness in the treatment of keloids. In this study, we investigated whether PDL treatments decreased transforming growth factor‐β1 (TGF‐β1)—induction and up‐regulation of matrix metalloproteinase (MMP) expression in keloid regression. Study Design/Materials and Methods Keloid tissues obtained from 10 patients with intra‐lesional or punch biopsies before and 7 days after PDL treatments [fluence per pulse was 10–18 J/cm 2 (mean 14.0 J/cm 2 )]. Immunohistochemical (IHC) staining of TGF‐β1 and MMP‐1 and MMP‐13 expressions in keloid tissue was performed. Western blot analysis of MMP‐1 and MMP‐13 expressions in extracellular matrix was evaluated. Results IHC staining indicated that expression of TGF‐β1 was significantly reduced in keloid tissues after PDL irradiation. MMP‐13 but not MMP‐1 expression on IHC staining significantly increased in extracellular matrix of keloid tissues after PDL treatment. Western blot analysis also showed MMP‐13 but not MMP‐1 significant increased in keloid tissues after PDL treatment. Conclusions Regression of keloids regressed after PDL treatments are associated with down‐regulation of TGF‐β1 expression and up‐regulation of MMP‐13 activity. Lasers Surg. Med. 36:38–42, 2005. © 2005 Wiley‐Liss, Inc.