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Effect of low‐power laser irradiation on protein synthesis and ultrastructure of human gingival fibroblasts
Author(s) -
Marques Márcia M.,
Pereira Aymann N.,
Fujihara Neusa A.,
Nogueira Fernando N.,
Eduardo Carlos P.
Publication year - 2004
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20008
Subject(s) - ultrastructure , irradiation , cytoplasm , laser , cell culture , chemistry , organelle , microbiology and biotechnology , biophysics , biology , anatomy , optics , physics , genetics , nuclear physics
Background and Objectives Low‐power lasers improve wound healing. Cell proliferation and protein secretion are important steps of this process. The aim of this study was to analyze both protein synthesis and ultrastructural morphology of human gingival fibroblasts irradiated by a low‐power laser. Study Design/Materials and Methods The cell line FMM1 was grown in nutritional deficit. Laser irradiation was carried out with a gallium–aluminum–arsenate (Ga–Al–As) diode laser (904 nm, 120 mW, energy density of 3 J/cm 2 ). The protein synthesis analysis and ultrastructural morphology of control (non‐irradiated) and irradiated cultures were obtained. Results There were changes in the structure of cytoplasm organelles of treated cells. The procollagen was not altered by the laser irradiation; however, there were a significant reduction of the amount of protein in the DMEM conditioned by irradiated cells. Conclusions Low‐power laser irradiation causes ultrastructural changes in cultured fibroblasts. We suggest that these alterations may lead to disturbances in the collagen metabolism. Lasers Surg. Med. 34:260–265, 2004. © 2004 Wiley‐Liss, Inc.