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Natural fluorescence of normal and neoplastic human colon: A comprehensive “ex vivo” study
Author(s) -
Bottiroli Giovanni,
Croce Anna C.,
Locatelli Donata,
Marchesini Renato,
Pignoli Emanuele,
Tomatis Stefano,
Cuzzoni Carolina,
Di Palma Silvana,
Dalfante Marco,
Spinelli Pasquale
Publication year - 1995
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900160107
Subject(s) - autofluorescence , ex vivo , pathology , submucosa , in vivo , stromal cell , fluorescence , biology , chemistry , medicine , optics , physics , microbiology and biotechnology
Background and Objective : A microspectrofluorometric analysis on “ex vivo” samples from normal tissue and adenocarcinoma of the human colon has been performed to characterize the histological, biochemical, and biophysical bases of the autofluorescence. Study Design/Materials and Methods : Differences between normal and tumor tissues are found that concern both the intensity distribution and spectral shape of the autofluorescence emission. The different pattern of the fluorescence intensity can be related to the histological organization of the tissue, and involves mainly the arrangement of the submucosa, the most fluorescent layer. Results : The most evident differences in the spectral shape found in the 480–580 nm range involve the stromal compartment, seem to be due to the presence of different fluorochromes, and are possibly related to the host response to the tumor. Conclusion : The nature and the extent of the autofluorescence modification between normal and tumor tissue in sections explain at least partly the evidence of the “in vivo” analysis and highlight the importance of excitation for full exploitation of the potentials of autofluorescence in diagnosis. © 1995 Wiley‐Liss, Inc.