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Effect of low level diode laser irradiation of human oral mucosa fibroblasts in vitro
Author(s) -
Loevschall Henrik,
ArenholtBindslev Dorthe
Publication year - 1994
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900140407
Subject(s) - irradiation , thymidine , andrology , microbiology and biotechnology , chemistry , in vitro , zoology , nuclear medicine , biology , biochemistry , medicine , physics , nuclear physics
The effects of low level laser (LLL) irradiation on the proliferation of human buccal fibroblasts were studied. A standardized LLL set‐up was developed (812 nm, 4.5 ± 0.5 mW/cm2). Cultures in petridishes were divided into eight groups (1 group served as control). On day 6 after seeding, routine growth medium was replaced with PBS for 1/2 hour. At the beginning of this period, LLL irradiation was performed for 0, 1, 3, 10, 32, 100, 316, or 1,000 seconds, respectively—corresponding to the radiant exposures 0, 4.5, 13.5, 45, 144, 450, 1,422, 4,500 mJ/cm2. Subsequently the cells received 3H‐dT in fresh medium for 16 hours DNA‐incorporation. Scintillations from tritium and total protein concentration per culture dish were determined. The individual 3H‐cpm/protein‐concentration ratios were calculated in % of control. Three experiments were performed (N = 151). Following LLL exposure the H‐cpm/protein ratio was increased with maximum cpm/protein ratio (132.5% ± 10.6% SEM) in the group receiving 450 mJ/cm2 (P < 0.03 nonparametric Kruskal Wallis one‐way ANOVA‐test). This study demonstrated an increased incorporation on tritiated thymidine in cultured human oral fibroblasts following LLL exposure and suggests that LLL irradiation can induce increased DNA Synthesis. © 1994 Wiley‐Liss, Inc.