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Effect of multidrug‐resistant P‐glycoprotein gene expression on chloroaluminum tetrasulfonate phthalocyanine concentration
Author(s) -
Frazier Donita L.,
Barnhill Mary Ann,
Lu Xiaochen,
Jones Emily,
Niemeyer Glenn,
Mishu Leslie,
Lothrop Clint D.
Publication year - 1993
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900130503
Subject(s) - phthalocyanine , multiple drug resistance , p glycoprotein , gene , gene expression , chemistry , glycoprotein , cancer research , microbiology and biotechnology , biology , biochemistry , organic chemistry , antibiotics
The effect of multidrug‐resistant P‐glycoprotein gene expression (MDR1) in 3T3 cells on cellular concentrations and cytotoxicity induced by the photodynamic agent chloroaluminum tetrasulfonate phthalocyanine (AISPc) was evaluated. 3T3 cells transfected with a retroviral vector expressing human MDR1 cDNA were resistant to colchicine. Resistant cells incubated with daunomycin accumulated only 40–50% of the quantity of daunomycin accumulated in control cells. Resistant cells incubated with daunomycin in the presence of verapamil had intracellular daunomycin concentrations approximately equal to control cells without verapamil. When these MDR1 3T3 cells were incubated with AISPc, cellular concentrations of AISPc did not differ between cells resistant to colchicine and those that were not. Similarly, there was little difference in cytotoxicity demonstrated by 51 Cromium release in the two cell lines exposed to AISPc and light (675 nm; 6 J/cm 2 ). This study suggests photodynamic therapy using AISPc may be a useful treatment modality for tumors in which the MDR1 P‐glycoprotein confers resistance to cancer chemotherapeutics. © 1993 Wiley‐Liss, Inc.