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Changes in oxidative metabolism of murine spleen following laser and superluminous diode (660–950 nm) irradiation: Effects of cellular composition and radiation parameters
Author(s) -
Karu T.,
Andreichuk T.,
Ryabykh T.
Publication year - 1993
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900130410
Subject(s) - irradiation , splenocyte , laser , chemistry , radiation , spleen , medicine , optics , physics , nuclear physics
Chemiluminescence (CL) of splenocytes of A/Sn mice was recorded after irradiation of the cells with various individual laser an superluminous diode probes at wavelengths from 660 to 950 nm (pulse repetition rates varying from 4 to 5,000 Hz) and at various doses. Laser radiation was found to increase or suppress the spontaneous CL of splenocytes suspension, the amplitude and the sign of the effect depending on the cellular composition of the samples. Direct correlations between the effect of laser radiation (per cent in changes of CL when irradiated at 820 nm, 1.1.10 3 J/m 2 , 292 Hz) and per cent of plasmacytes (r = 0.743, P<0.001), neutrophils (r = 0.650, P<0.001) as well as myelocytes and metamyelocytes (r = 0.505, P<0.01) were established. The correlation with per cent of lymphocytes (r = −0.590, P<0.001) was found to be a reverse one. Dependence of the irradiation effects on dose, pulse repetition rate, and wavelength are presented. © 1993 Wiley‐Liss, Inc.