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Fluorospectral study of the rat brain and glioma in vivo
Author(s) -
Tsai JuiChang,
Kao MingChien,
Hsiao YiYun
Publication year - 1993
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900130309
Subject(s) - glioma , autofluorescence , in vivo , hematoporphyrin , pathology , brain tissue , brain tumor , fluorescence , medicine , chemistry , biology , biomedical engineering , cancer research , photodynamic therapy , optics , physics , microbiology and biotechnology , organic chemistry
An animal model of cerebral glioma was utilized by implanting C6 glioma cells into the brains of adult Wistar rats. Once tumors developed to 7–12 mm in diameter, we conducted continuous fluorimetry monitoring of glioma up to 24 hours using a fibre‐optic system connected to an intensified multichannel photodetector after an intravenous injection of hematoporphyrin derivative (HPD) into the rats. The intensity of the fluorescence in normal brain reached a plateau 6 hours after intravenous injection of HPD while that in glioma reached a plateau 80 minutes after injection. These fluorescence intensities of glioma, brain adjacent to tumor (BAT), and surrounding normal brain were measured in vivo 24 hours after intravenous administration of 5 mg/kg of HPD. The ratio of fluorescence intensities between glioma and brain was 6.1 while the ratio between BAT and brain was 3.9. There were no obvious differences in shapes between the spectra of the natural fluorescence (autofluorescence) of rat glioma and brain but the intensity of autofluorescence was much weaker in glioma. There are many problems in spectroscopic studies of biological tissues in vivo. It cannot be overemphasized that very strict criteria must be applied in order to get accurate data. Fluorescence from HPD administration may be used to discriminate tumor tissue from surrounding normal brain tissue during operation if the measuring conditions could be kept constant. It is important to understand the photospectral properties of glioma and brain tissue in order to get the most benefits in clinical application of light‐induced fluorescence or photoradiation therapy. © 1993 Wiley‐Liss, Inc.