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Photoinhibition of smooth muscle cell migration: Potential therapy for restenosis
Author(s) -
Deckelbaum Lawrence I.,
Scott John J.,
Stetz Mark L.,
O'Brien Kenneth M.,
Sumpio Bauer E.,
Madri Joseph A.,
Bell Leonard
Publication year - 1993
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900130104
Subject(s) - neointima , restenosis , cell migration , photoinhibition , irradiation , cell growth , cell , in vivo , chemistry , biophysics , anatomy , biology , medicine , biochemistry , photosynthesis , physics , microbiology and biotechnology , photosystem ii , stent , nuclear physics
Evidence from animal, autopsy, and atherectomy studies demonstrates that migration and proliferation of smooth muscle cells of medial origin result in neointima formation and decreased luminal cross‐sectional area. The purpose of this study was to evaluate whether low energy light irradiation can inhibit smooth muscle cell migration and therefore potentially reduce the degree of neointima formation and the incidence of restenosis. The migration kinetics of bovine aortic smooth muscle cell monolayers were examined using a fence assay. The effect on smooth muscle cell migration of irradiation with monochromatic light at wavelengths ranging from 400 to 700 nm was compared to the migration of cells irradiated with broadband white light or maintained in the dark. Wavelength specific photoinhibition of smooth muscle cell migration was observed; 594–600 nm light reproducibly inhibited migration by 12–29% ( P < 0.05). Migration rate was significantly reduced following daily radiant exposures of 1.0 J/cm 2 as well as following a single radiant exposure of 0.09 or 0.9 J/cm 2 . The decrease in migration was not associated with any change in cell proliferation or [ 3 H] thymidine incorporation. We conclude that 594–600 nm light inhibits smooth muscle cell migration in vitro and may potentially be used in vivo to decrease fibrointimal thickening following arterial injury. This application of photoinhibition may be useful in retarding restenosis following angioplasty. © 1993 Wiley‐Liss, Inc.