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Laser Raman spectrum of calcified human aorta
Author(s) -
Klug Dennis D.,
Singleton Donald L.,
Walley Virginia M.
Publication year - 1992
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900120105
Subject(s) - raman spectroscopy , aorta , materials science , laser , excitation wavelength , wavelength , excitation , spectral line , fluorescence , analytical chemistry (journal) , optics , chemistry , optoelectronics , medicine , chromatography , physics , electrical engineering , astronomy , engineering
Raman spectra of highly calcified areas of areas of human aorta were obtained using long wavelength excitation at 740 nm minimize background fluorescence interference. Raman spectra resembling that of hydroxyapatite were obtained for segments of highly calcified aorta that were thawed frozen samples, from which the surface layer had been removed, or were dired. These are the first reported spectra obtained in air or in normal saline solution showing the hydroxyapatite peak in calcified plaque for samples which were untreated except for freezing, which is encouraging for the application of Raman spectroscopy as a method of detection of plaque during laser angioplasty. Spectra obtained from calcified aorta using excitation at 514.5 nm did not show evidence of the hydroxykapatite band because of the severe interference by background fluorescence generated at this wave length.