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Hyperthermia potentiates the effects of aluminum phthalocyanine tetrasulfonate‐mediated photodynamic toxicity in human malignant and normal cell lines
Author(s) -
Glassberg Edward,
Lewandowski Lawrence,
Halcin Cynthia,
Lask Gary,
Uitto Jouni
Publication year - 1991
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900110508
Subject(s) - hyperthermia , photodynamic therapy , cell culture , fibrosarcoma , toxicity , cancer research , in vitro , chemistry , melanoma , cell , pathology , medicine , biology , biochemistry , organic chemistry , genetics
The purpose of this study was to examine the effects of photodynamic therapy utilizing aluminum phthalocyanine tetrasulfonate in vitro on several human malignant and normal cell types, with or without hyperthermia. Cells examined included normal skin fibroblasts, HT‐1080 fibrosarcoma cells, SCC‐25 (squamous cell carcinoma) and malignant melanoma cells. An argon‐pumped continuous wave tunable dye laser at 675 nm was used as the light source, hyperthermia groups were heated to 42.5°C, and radioisotope incorporation was used to measure DNA and protein synthesis as toxicity assays. Results showed an energy‐dose, and AlPcS‐concentration dependent toxicity in all cell lines examined, with moderate selectivity toward malignant cells. Hyperthermia lone was slightly toxic in melanomas and HT‐1080 cell lines but had no effect in normal fibroblasts or SCC‐25 cells. Hyperthermia synergistically potentiated the effects of PDT in all cell lines, and the combined modality was significantly more toxic in all malignant cell lines compared with normal cells. Thus, addition of hyperthermia to PDT protocols may enhance the efficacy of this treatment modality in vitro.