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Direct comparison of in‐vitro and in‐vivo photofrin‐II mediated photosensitization using a pulsed KTP pumped dye laser and a continuous wave argon ion pumped dye laser
Author(s) -
Ferrario Angela,
Rucker Natalie,
Ryter Stefan W.,
Doiron Daniel R.,
Gomer Charles J.
Publication year - 1991
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900110504
Subject(s) - photobleaching , laser , photosensitizer , dye laser , photodynamic therapy , in vivo , continuous wave , argon , chemistry , materials science , fluorescence , biophysics , optics , optoelectronics , photochemistry , physics , microbiology and biotechnology , organic chemistry , biology
A pulsed KTP pumped dye laser (25 kHz repetition rate and 470 nsec pulse width) has been compared to a continuous wave argon ion pumped dye laser as the source of 630 nm light during in‐vitro and in‐vivo Photofrin‐II mediated photosensitization studies. Individual experiments documented the effectiveness of each laser system on a) photosensitizer induced cytotoxicity and induction of stress protein synthesis using Chinese hamster fibroblasts; b) photobleaching of Photofrin‐II in aqueous solution; c) Photofrin II mediated photosensitization of normal mouse skin; d) Photofrin II mediated photodynamic therapy of a mouse mammary carcinoma; and e) tumor temperature levels generated during laser exposure. Comparable results were obtained for both laser systems in all experiments.