Premium
In vitro production of viable bacteriophage in carbon dioxide and argon laser plumes
Author(s) -
Matchette Stephanie L.,
Faaland Robert W.,
Royston David D.,
Ediger Marwood N.
Publication year - 1991
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900110411
Subject(s) - argon , plume , laser , bacteriophage , absorption (acoustics) , substrate (aquarium) , laser ablation , carbon dioxide , chemistry , materials science , analytical chemistry (journal) , environmental chemistry , optics , biology , escherichia coli , physics , meteorology , composite material , gene , ecology , biochemistry , organic chemistry
Both CO 2 and argon laser ablation of an agar substrate containing high titres of bacteriophage ϕX174 create plumes which disperse viable phage particles. Irradiances at the beam impact site ranged from 73 to 215 W/cm 2 for the CO 2 laser and from 40 to 227 W/cm 2 for the argon laser. To increase the absorption of argon laser radiation, oxidized hemoglobin was added to the target material. Plume‐borne viable phage were observed to be associated with particles large enough to settle out from the plume within 100 mm of the beam impact site. The ratio of the number of dispersed viable phage to the number of viable phage potentially dispersible by a single, 1‐second laser exposure was on the order of 10 −6 to 10 −5 .