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Dependence of subcellular damage on various laser beam parameters
Author(s) -
Oyog Shirley S.,
Smith Michael J.,
van de Merwe Willem P.
Publication year - 1991
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900110410
Subject(s) - laser , lesion , optics , materials science , argon , chemistry , pathology , physics , medicine , organic chemistry
A cw argon laser operating at the multiline UV TEM 00 mode was used to create lesions in the nucleoli of fibroblast cells. Laser powers emitted through the objective were varied from 5–8 mW and laser pulse durations ranged from 50 to 600 ms. Our study showed lesion diameters ranging from 0.7 μm to 2.9 μm and an average lesion diameter of 1.77 μm. All cells were exposed to at most a laser spotsize of 2.2 μm diameter and for a laser spotsize of this diameter, we calculated that the central 18.3% of the total energy was in the central area of 0.7 μm diameter. We also found that as little as 0.3 mJ of energy could produce a lesion. No absolute threshold correlation was found between the size or type of lesion and the laser parameters used.