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Photodynamic therapy for rat pituitary tumor in vitro and in vivo using pheophorbide‐a and white light
Author(s) -
Yano Takashi,
Uozumi Tohru,
Kawamoto Keiichi,
Mukada Kazutoshi,
Onda Jun,
Ito Akihiro,
Fujimoto Nariaki
Publication year - 1991
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900110212
Subject(s) - pheophorbide a , in vivo , white light , in vitro , photodynamic therapy , white (mutation) , medicine , endocrinology , chemistry , biology , biochemistry , materials science , microbiology and biotechnology , organic chemistry , optoelectronics , gene
This is the first report on the use of photodynamic therapy (PDT) for rat pituitary tumor in vivo. Rat pituitary tumor (GH 3 ) cells were cultured, GH 3 tumor was subcutaneously implanted in nude mice, and pheophorbide‐a (Ph‐a) and white light were prepared. Photocytotoxicity proportional to Ph‐a concentration, intensity of irradiation, and incubation time was observed in vitro. Despite the delay in the disappearance of Ph‐a from the tumor, Ph‐a in the pituitary gland rapidly decreased after intravenous administration in vivo. Through PDT, the tumor grossly disappeared, the plasma levels of rat growth hormone secreted from the tumor also remarkably decreased, and the development of giantism was inhibited. These results indicate that PDT is effective against rat pituitary tumor.