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In vitro production of viable bacteriophage in a laser plume
Author(s) -
Ediger M. N.,
Matchette L. S.
Publication year - 1989
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900090315
Subject(s) - photoablation , laser , bacteriophage , plume , ablation , laser ablation , in vitro , optics , chemistry , materials science , analytical chemistry (journal) , escherichia coli , physics , chromatography , excimer laser , medicine , biochemistry , gene , thermodynamics
Bacteriophage ϕX 174, present at high concentrations in an agar overlay in vitro system, were used as a target for pulsed 2.94 μm (Er:YAG) laser ablation. In this preliminary experiment, the potential for transport of viable viruses in the photoablation plume was investigated for laser energy densities of approximately 3.5 J/cm 2 per pulse in exposures of 75–600 laser pulses. Transport of relatively few numbers of viable viruses from the ablation site to the plate assay detector, a distance of approximately 2 cm, was observed.