In vitro production of viable bacteriophage in a laser plume | Zendy

Ediger M. N. | Zendy; Matchette L. S. | Zendy

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In vitro production of viable bacteriophage in a laser plume

Author(s) -

Ediger M. N.,

Matchette L. S.

Publication year - 1989

Publication title -

lasers in surgery and medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.888

H-Index - 112

eISSN - 1096-9101

pISSN - 0196-8092

DOI - 10.1002/lsm.1900090315

Subject(s) - photoablation , laser , bacteriophage , plume , ablation , laser ablation , in vitro , optics , chemistry , materials science , analytical chemistry (journal) , escherichia coli , physics , chromatography , excimer laser , medicine , biochemistry , gene , thermodynamics

Bacteriophage ϕX 174, present at high concentrations in an agar overlay in vitro system, were used as a target for pulsed 2.94 μm (Er:YAG) laser ablation. In this preliminary experiment, the potential for transport of viable viruses in the photoablation plume was investigated for laser energy densities of approximately 3.5 J/cm 2 per pulse in exposures of 75–600 laser pulses. Transport of relatively few numbers of viable viruses from the ablation site to the plate assay detector, a distance of approximately 2 cm, was observed.

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