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New method of photosensitizer accumulation for photodynamic therapy in an experimental liver tumor
Author(s) -
Nishiwaki Yoshiro,
Nakamura Satoshi,
Sakaguchi Shukichi
Publication year - 1989
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900090308
Subject(s) - photosensitizer , photodynamic therapy , chemistry , medicine , photochemistry , organic chemistry
A new sensitizing method of photodynamic therapy for malignant tumors and its effects was studied. Prepared for the study were pheophorbide a (Phd), dissolved in an oily contrast medium, Lipiodol (LPD‐Phd), and water‐soluble pheophorbide a (WS Phd) as sensitizers, and VX‐2 tumor in rabbit livers. The Phd distribution was compared after intraarterial (i.a.) administration of LPD‐Phd or W‐S Phd and intravenous (i.v.) administration of W‐S Phd. Phd was extracted with methanol at 24 h after injection, and the supernatant absorbance was measured at 670 nm by spectrophotometry. The tumor showed higher values of Phd than did the liver with LPD‐Phd i.a. and W‐S Phd i.a. ( P < .01). Conversely, the tumor accumulated less Phd than did the liver with W‐S Phd i.v. ( P < .05). We subsequently produced severe photodestruction in a Walker tumor in a Sprague‐Dawley rat liver with slight damage to adjacent liver tissue using LPD‐Phd i.a. and Nd‐YAG dye laser irradiation at 670 nm. The intraarterial administration of a photosensitizer may make it possible to treat liver tumors by photodynamic therapy.