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In vitro photosensitization II. An electron microscopy study of cellular destruction with mono‐ L ‐aspartyl chlorin e 6 and photofrin II
Author(s) -
Roberts W. Gregory,
Liaw L.H. L.,
Berns Michael W.
Publication year - 1989
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900090204
Subject(s) - transmission electron microscopy , chlorin , irradiation , chemistry , electron microscope , in vitro , fluorescence microscope , biophysics , fluorescence , microscopy , mitochondrion , scanning electron microscope , photosensitizer , photochemistry , biology , biochemistry , materials science , pathology , nanotechnology , medicine , optics , physics , nuclear physics , composite material
Primary sites of subcellular destruction with photofrin II (PfII) and mono‐ L ‐aspartyl chlorin e 6 (MACE) were determined by transmission electron microscopy (TEM). Potorous tridactylus (PTK 2 ) cells were grown in Rose chambers and incubated for 24 hr with a sensitizer concentration sufficient to provide 100% mortality. Cells were irradiated with laser light and fixed and processed for electron microscopy at various times post‐irradiation. The results indicate that PfII initially destroys mitochondria, whereas MACE destroys lysosomes. Both conclusions are consistent with fluorescence subcellular localization studies.