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Biological effect of He‐Ne laser: Investigations on functional and micromorphological alterations of cell membranes, in vitro
Author(s) -
Kubasova Tamara,
Somosy Zoltán,
Unk Péter,
Kókai András,
Kovács Lajos
Publication year - 1984
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900040411
Subject(s) - cell , irradiation , biophysics , in vitro , fibroblast , membrane , ultrastructure , cell membrane , chemistry , laser , electron microscope , biology , anatomy , biochemistry , optics , physics , nuclear physics
Abstract A 1‐J/cm 2 dose of single laser‐irradiation applied on primary human embryo fibroblast culture was not followed either by functional or micromorphological alterations of cell surfaces. This dose, however, applied four times with 24‐hour intervals changed the functional conditions as well as surface charges of cell membranes. As detection methods, radioactive glucosamine uptake, lectin, and cationized ferritin‐binding techniques were applied. At the same time, the scanning and transmission electron microscopy of laser‐irradiated cells did not reveal any micromorphological or ultrastructural alterations. The effect of a 5‐J/cm 2 dose did not differ from that of 1 J/cm 2 . It is suggested that laser radiation‐induced circumstances on cell surfaces can contribute to the strength of cell‐to‐cell contacts, i.e., to the stimulation of epithelization experienced in the clinical gynecol‐ ogic practice.