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Time‐resolved fluorescence microscopy of hematoporphyrin‐derivative in cells
Author(s) -
Docchio F.,
Ramponi R.,
Sacchi C.A.,
Bottiroli G.,
Freitas I.
Publication year - 1982
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1900020103
Subject(s) - hematoporphyrin , fluorescence , fluorescence microscope , microscopy , cytoplasm , hela , biophysics , chemistry , derivative (finance) , cell , materials science , optics , biology , photodynamic therapy , biochemistry , physics , organic chemistry , financial economics , economics
Abstract This work presents measurements of time‐resolved fluorescence microscopy of hematoporphyrin‐derivative (HpD) in single cells of mice tissue (both tumor and normal cells), in HeLa cells, and in solution. The measurements were performed using a pulsed‐laser microfluorometer with high spatial and temporal resolution. In agreement with the results obtained with other techniques, it has been found that the tumor cells examined present an HpD uptake about five times higher than that of the normal cells of the corresponding tissue and that, within a cell, HpD becomes localized mainly in the cytoplasm. It has also been found that the fluorescence decay time is different in cells as compared with solution, and that the presence of HpD stabilizes cell auto‐fluorescence. These results are discussed.