Premium
Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence *
Author(s) -
AlmeidaLopes Luciana,
Rigau Josepa,
Amaro Zângaro Renato,
GuidugliNeto João,
Marques Jaeger Márcia Martins
Publication year - 2001
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1107
Subject(s) - cell growth , fluence , irradiation , fetal bovine serum , fibroblast , laser , cell culture , low level laser therapy , in vitro , andrology , materials science , wound healing , chemistry , biomedical engineering , biophysics , medicine , immunology , biology , optics , laser therapy , biochemistry , physics , nuclear physics , genetics
Background and Objective: The low level laser therapy (LLLT) has been used in Dentistry to improve wound healing. In order to analyse the effect of LLLT on the in vitro proliferation of gingival fibroblasts we developed a primary culture of human gingival fibroblasts. Study Design/Materials and Methods: The cell line named LMF was grown in Dulbecco's Modified Eagle's medium (DME) with either 5% (nutritional deficit) or 10% fetal bovine serum (fbs). Laser irradiation was carried out with diode lasers with the following wavelengths: 670 nm (L1), 780 nm (L2), 692 nm (L3), and 786 nm (L4). The fluence was fixed in 2 J/cm 2 . For growth analysis, control (not irradiated) and treated cultures (irradiated) were plated in 60 mm diameter culture dishes for 12 h before the irradiation. Results: We found that cells cultured in nutritional deficit condition grown in medium supplemented by only 5% fbs presented a cell proliferation rate significantly smaller that cell grown in ideal culture conditions (10% fbs). However, when irradiated, cells in nutritional deficit presented cell growth similar or higher than that of control cells grown in ideal culture conditions. Using the same fluence, the infrared laser induced a higher cell proliferation than visible laser when the power outputs were different. However, lasers of equal power output presented similar effect on cell growth independently of their wavelengths. Conclusions: The LLLT acts by improving the in vitro fibroblast proliferation and a smaller laser exposure time results in higher proliferation. Lasers Surg. Med. 29:179–184, 2001. © 2001 Wiley‐Liss, Inc.