Premium
Fluorescence spectroscopy of epithelial tissue throughout the dysplasia‐carcinoma sequence in an animal model: Spectroscopic changes precede morphologic changes
Author(s) -
Coghlan Lezlee,
Utzinger Urs,
RichardsKortum Rebecca,
Brookner Carrie,
Zuluaga Andres,
GimenezConti Irma,
Follen Michele
Publication year - 2001
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1078
Subject(s) - pathology , dysplasia , carcinoma , fluorescence spectroscopy , animal model , biology , epithelial dysplasia , fluorescence , anatomy , chemistry , medicine , optics , physics , endocrinology
Background and Objective The hamster cheek pouch carcinogenesis model, using chronic treatments of dimethylbenz[α]anthracene (DMBA) was used as a model system to investigate changes in epithelial tissue autofluorescence throughout the dysplasia‐carcinoma sequence. Study Design/Materials and Methods Fluorescence emission spectra were measured weekly from 42 DMBA‐treated animals and 20 control animals at 337, 380, and 460 nm excitation. A subset of data in which histopathology was available was used to develop diagnostic algorithms to separate neoplastic and non‐neoplastic tissue. The change in fluorescence intensity over time was examined in all samples at excitation‐emission wavelength pairs identified as diagnostically useful. Results Algorithms based on autofluorescence can separate neoplastic and non‐neoplastic tissue with 95% sensitivity and 93% specificity. Greatest contributions to diagnostic algorithms are obtained at 380 nm excitation, and 430, 470, and 600 nm emission. Changes in fluorescence intensity are apparent as early as 3 weeks after initial treatment with DMBA, whereas morphologic changes associated with dysplasia occur on average at 7.5–12.5 weeks after initial treatment. Conclusions Fluorescence spectroscopy provides a potential tool to identify biochemical changes associated with dysplasia and hyperplasia, which precede morphologic changes observed in histologically stained sections. Lasers Surg. Med. 29:1–10, 2001. © 2001 Wiley‐Liss, Inc.