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Histologic evaluation of skin damage after overlapping and nonoverlapping flashlamp pumped pulsed dye laser pulses: A study on normal human skin as a model for port wine stains
Author(s) -
Koster Petra H.L.,
van der Horst Chantal M.A.M.,
van Gemert Martin J.C.,
van der Wal Allard C.
Publication year - 2001
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.1036
Subject(s) - port wine , human skin , dye laser , laser , homogeneous , pulse (music) , materials science , irradiation , port wine stain , connective tissue , optics , biomedical engineering , pathology , medicine , surgery , genetics , physics , detector , nuclear physics , biology , thermodynamics
Background and Objective In the treatment of port wine stains (PWS) with the flashlamp pumped pulsed dye laser (FPPDL), no consensus exists about overlapping of pulses. The advantage of overlapping pulses is homogeneous lightening of the PWS; the risk is redundant tissue damage. The aim of this study was to determine the histopathologic effect on human skin of pulsed dye laser pulses with various degrees of overlap, with normal human skin as a model for PWS. Study Design/Materials and Methods Eighteen healthy white volunteers were irradiated with pulsed dye laser pulses with increasing radiant exposure and with different degrees of overlap. Biopsy samples were taken and histologically analysed. Results Overlapping of pulses on normal human skin enhances depth of vascular damage with approximately 30%. Adjacent pulses also show this effect. We found no histologic signs of serious damage to epidermis or dermal connective tissue by using radiant exposure levels of 6–8 J/cm 2 , regardless of pulse application. Conclusions Reasoning that the mechanism of tissue injury is comparable for normal and PWS skin, we conclude that it is safe to treat PWS with overlapping FPPDL pulses to achieve homogeneous lightening. Lasers Surg. Med. 28:176–181, 2001. © 2001 Wiley‐Liss, Inc.