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Light emitting diode irradiation protect against the amyloid beta 25–35 induced apoptosis of PC12 cell in vitro
Author(s) -
Duan Rui,
Zhu Ling,
Liu Timon ChengYi,
Li Yan,
Liu Jiang,
Jiao Jianling,
Xu Xiaoyang,
Yao Libo,
Liu Songhao
Publication year - 2003
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.10216
Subject(s) - apoptosis , dna fragmentation , programmed cell death , fragmentation (computing) , irradiation , beta (programming language) , microbiology and biotechnology , uvb induced apoptosis , amyloid beta , in vitro , chemistry , cell , cancer research , biology , biochemistry , caspase , physics , ecology , computer science , nuclear physics , programming language , peptide
Background and Objectives Whether light emitting diode (LED) irradiation has effects on “β‐amyloid (Aβ) induced apoptosis,” a leading hypothesis of the cause of Alzheimer's disease (AD), or not? Study Design/Materials and Methods Monolayer cell cultures of PC12 were subjected to Aβ or/and LED irradiation at various intensity. Cell apoptosis was confirmed by morphological criteria, DNA fragmentation assay, and FAScan flow cytometer assay. Results Treatment of the cells with LED irradiation significantly diminished Aβ induced apoptosis within 24 hours. Conclusions The LED irradiation, when utilized at power of 0.9 W/m 2 and 60 minutes has significantly diminished Aβ induced apoptosis of PC12 cells. Lasers Surg. Med. 33:199–203, 2003. © 2003 Wiley‐Liss, Inc.