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Autofluorescence characteristics of healthy oral mucosa at different anatomical sites
Author(s) -
de Veld Diana C.G.,
Skurichina Marina,
Witjes Max J.H.,
Duin Robert P.W.,
Sterenborg Dick J.C.M.,
Star Willem M.,
Roodenburg Jan L.N.
Publication year - 2003
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.10185
Subject(s) - autofluorescence , excitation wavelength , principal component analysis , oral mucosa , dorsum , tongue , fluorescence , chemistry , pathology , spectroscopy , nuclear medicine , nuclear magnetic resonance , optics , medicine , anatomy , physics , artificial intelligence , computer science , quantum mechanics
Background and Objectives Autofluorescence spectroscopy is a promising tool for oral cancer detection. Its reliability might be improved by using a reference database of spectra from healthy mucosa. We investigated the influence of anatomical location on healthy mucosa autofluorescence. Study Design/Materials and Methods Spectra were recorded from 97 volunteers using seven excitation wavelengths (350–450 nm), 455–867 nm emission. We studied intensity and applied principal component analysis (PCA) with classification algorithms. Class overlap estimates were calculated. Results We observed differences in fluorescence intensity between locations. These were significant but small compared to standard deviations (SD). Normalized spectra looked similar for locations, except for the dorsal side of the tongue (DST) and the vermilion border (VB). Porphyrin‐like fluorescence was observed frequently, especially at DST. PCA and classification confirmed VB and DST to be spectrally distinct. The remaining locations showed large class overlaps. Conclusions No relevant systematic spectral differences have been observed between most locations, allowing the use of one large reference database. For DST and VB separate databases are required. Lasers Surg. Med. 32:367–376, 2003. © 2003 Wiley‐Liss, Inc.