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Effects of low intensity infrared laser radiation on the water transport in the isolated toad urinary bladder
Author(s) -
Franco Wilson,
Leite Renner S.,
Parizotto Nivaldo A.
Publication year - 2003
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.10166
Subject(s) - vasopressin , water transport , toad , chemistry , laser , biophysics , adenylate kinase , endocrinology , bufo marinus , cyclic adenosine monophosphate , medicine , adenosine , water flow , biochemistry , biology , optics , enzyme , physics , receptor , environmental engineering , engineering
Background and Objectives The aim of this work was to study the effects of low intensity laser radiation on water transport in the toad bladder in vitro. Study Design/Materials and Methods The water flow through the membrane was measured gravimetrically in bag preparations of the membrane. Results Laser radiation did not alter the water transport in the presence nor in the absence of vasopressin. In contrast, when the hemibladders were previously treated with vasopressin, the laser decreased by approximately 33.70% arginine‐vasopressin (AVP)‐mediated water transport. Laser radiation increased 3′5′‐cyclic adenosine monophosphate (3′5′‐cAMP) mediated water transport by approximately 23%. The association of laser radiation with indomethacin (IND) did not affect AVP‐mediated water transport. Conclusions This data suggests that the laser may have two effects on AVP‐mediated water transport: one inhibitory effect on 3′5′‐cAMP synthesis by inhibiting the adenylate cyclase complex and another stimulatory effect by inhibiting nucleotide‐phosphodiesterase activity. Our results also indicate that the laser does not interfere in the prostaglandins biosynthesis induced by AVP. Lasers Surg. Med. 32: 299–304, 2003. © 2003 Wiley‐Liss, Inc.