Premium
Measurement of adhesive forces between S. epidermidis and fibronectin‐coated surfaces using optical tweezers
Author(s) -
Simpson Kathryn Hicks,
Bowden M. Gabriela,
Höök Magnus,
Anvari Bahman
Publication year - 2002
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.10070
Subject(s) - optical tweezers , adhesion , fibronectin , adhesive , chemistry , biophysics , materials science , cell adhesion , nanotechnology , coating , layer (electronics) , composite material , optics , cell , biochemistry , biology , physics
Background and Objectives Biomaterial‐mediated infection, a common cause of medical device failure, is initiated by bacterial adhesion to an adsorbed protein layer on the implant surface. This adhesion is thought to be mediated by specific molecules present on the bacterial cell surface. Optical tweezers can be used to measure the adhesive force between a single bacterium and a protein‐coated surface. Study Design/Materials and Methods Using optical tweezers, a bacterium was trapped and brought in contact with a 10‐μm diameter polystyrene microsphere coated with fibronectin. The minimum force required to detach the cell from the bead was determined over a range of fibronectin concentrations and contact times. Results The detachment forces were integer multiples of an 18‐pN base value that was independent of contact time and coating concentration; we propose that the variation in force is related to the number of bonds formed. Conclusions These experiments demonstrate that optical tweezers can be used to investigate the adhesion of individual bacteria to surfaces. The results suggest that S. epidermidis has surface proteins capable of binding fibronectin. Lasers Surg. Med. 31:45–52, 2002. © 2002 Wiley‐Liss, Inc.