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Photoeradication of Helicobacter pylori using 5‐aminolevulinic acid: Preliminary human studies
Author(s) -
WilderSmith Clive H.,
WilderSmith Petra,
Grosjean Pierre,
van den Bergh Hubert,
Woodtli Alain,
Monnier Philippe,
Dorta Gian,
Meister Friedrich,
Wagnières Georges
Publication year - 2002
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.10066
Subject(s) - helicobacter pylori , in vivo , antrum , gastritis , white light , rapid urease test , laser light , irradiation , gastroenterology , medicine , gastric mucosa , stomach , pathology , biology , laser , materials science , optics , physics , microbiology and biotechnology , optoelectronics , nuclear physics
Background and Objectives Helicobacter pylori (HP) is an endemic pathogenic bacterium causing gastritits and gastroduodenal ulceration in humans and is linked to the development of gastric malignancies. These first human in vivo studies investigated the photoeradication of HP using laser and white light. Study Design/Materials and Methods In 13 HP‐positive volunteers, a zone of gastric antrum was irradiated with laser (410 nm, 50 J/cm 2 ) or endoscopic white light (10 J/cm 2 ) 45 minutes after oral 5‐aminolevulinic acid (5‐ALA) 20 mg/kg. HP‐eradication was assessed by biopsy urease test and HP‐culture from irradiated and control zones 5 minutes, 4 and 48 hours post‐irradiation. Results A maximum eradication effect was achieved at 4 hours post‐irradiation when 85% of biopsies in the monochromatic and 66% in the white light exposed zones, and 58 and 33% in the respective control zones were HP‐negative. Conclusions HP numbers were greatly reduced following exposure to 5‐ALA and either laser or white light in vivo. Photoeradication appears feasible, but further light dosimetry and the development of convenient application methods is required. Lasers Surg. Med. 31:18–22, 2002. © 2002 Wiley‐Liss, Inc.