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Enhanced adenoviral‐vector mediated gene transfer using human albumin solder
Author(s) -
Rivera Arnold DC,
Walker Charles N.,
Bleustein Clifford,
Choi Benjamin,
Poppas Dix P.,
Felsen Diane
Publication year - 2002
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.10040
Subject(s) - in vivo , viral vector , genetic enhancement , wound healing , transduction (biophysics) , in vitro , adenoviridae , albumin , gene transfer , microbiology and biotechnology , chemistry , biology , gene , immunology , biochemistry , recombinant dna
Abstract Background and Objective Laser tissue welding with human albumin solder (HAS) has been used as an alternative method of wound closure. Adenoviral vectors have been used to introduce various cytokine genes into wounds to accelerate wound closure. In the present study, we were interested in the effect of HAS on adenoviral vector transfer of the β‐ galactosidase (β‐ gal ) gene in vitro and in vivo. Study Design/Materials and Methods 3T3 fibroblasts were used to study the effect of HAS on β‐ gal gene transfer in vitro. The presence of β‐ gal was determined by Western blot, and its activity by a colorimetric assay. A punch biopsy model of wound healing in pigs was used for in vivo experiments. Results HAS increased the efficiency of adenoviral‐mediated β‐gal transduction and stabilized the adenovirus at room temperature. HAS protected adenovirus from inactivation by laser, both in vitro and in vivo. Conclusions HAS may stabilize adenoviral vectors to deliver cytokine genes in future wound healing experiments. Lasers Surg. Med. 30:313–319, 2002. © 2002 Wiley‐Liss, Inc.