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Neuroimaging: High‐Speed Large‐Field Multifocal Illumination Fluorescence Microscopy (Laser Photonics Rev. 14(2)/2020)
Author(s) -
Chen Zhenyue,
Mc Larney Benedict,
Rebling Johannes,
DeánBen Xosé Luis,
Zhou Quanyu,
Gottschalk Sven,
Razansky Daniel
Publication year - 2020
Publication title -
laser and photonics reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.778
H-Index - 116
eISSN - 1863-8899
pISSN - 1863-8880
DOI - 10.1002/lpor.202070013
Subject(s) - microscopy , optics , photonics , neuroimaging , fluorescence lifetime imaging microscopy , depth of field , focus (optics) , grating , materials science , fluorescence microscope , light sheet fluorescence microscopy , laser , optical microscope , fluorescence , physics , neuroscience , scanning confocal electron microscopy , biology , scanning electron microscope
In article number 1900070, Daniel Razansky and co‐workers report on a novel optical microscopy concept termed large‐field multifocal illumination (LMI) microscopy based on a beam‐splitting grating and an acousto‐optic deflector synchronized with a high speed camera. The method offers a unique combination between centimeter scale field‐of‐view, enhanced depth of focus, and real‐time imaging performance. The cover shows an artistic representation of structural and functional imaging of the mouse brain.