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Talbot holographic illumination nonscanning (THIN) fluorescence microscopy
Author(s) -
Luo Yuan,
Singh Vijay Raj,
Bhattacharya Dipanjan,
Yew Elijah Y. S.,
Tsai JuiChang,
Yu SungLiang,
Chen HsiHsun,
Wong JauMin,
Matsudaira Paul,
So Peter T. C.,
Barbastathis George
Publication year - 2014
Publication title -
laser and photonics reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.778
H-Index - 116
eISSN - 1863-8899
pISSN - 1863-8880
DOI - 10.1002/lpor.201400053
Subject(s) - holography , optical sectioning , optics , focus (optics) , materials science , microscopy , confocal , confocal microscopy , fluorescence lifetime imaging microscopy , digital holographic microscopy , fluorescence , physics
Optical sectioning techniques offer the ability to acquire three‐dimensional information from various organ tissues by discriminating between the desired in‐focus and out‐of‐focus (background) signals. Alternative techniques to confocal, such as active structured illumination, exist for fast optically sectioned images, but they require individual axial planes to be imaged consecutively. In this article, an imaging technique (THIN), by utilizing active Talbot illumination in 3D and multiplexed holographic Bragg filters for depth discrimination, is demonstrated for imaging in vivo 3D biopsy without mechanical or optical axial scanning.