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Back Cover Picture: Laser & Photon. Rev. 3(1‐2)/2009
Publication year - 2009
Publication title -
laser and photonics reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.778
H-Index - 116
eISSN - 1863-8899
pISSN - 1863-8880
DOI - 10.1002/lpor.200990001
Subject(s) - biotinylation , streptavidin , polyethylene glycol , photonics , laser , molecule , biotin , cover (algebra) , nanotechnology , chemistry , materials science , optics , physics , optoelectronics , biochemistry , organic chemistry , mechanical engineering , engineering
Anchoring proteins to surfaces for single‐molecule optical‐trapping experiments. Biotinylated‐BSA, bovine serum albumin (grey) with an attached biotin molecule, can be adsorbed to glass. When the biotin (red dot) is correctly positioned, it is bound by streptavidin (green). Since streptavidin has four binding sites, it can also bind to the biotinylated helicase. Polyethylene glycol (PEG) (yellow) is covalently linked to a cover glass. A fraction of the PEG molecules are terminated in biotin which allows for a controlled density of streptavidin and thus the helicase.