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Optical traps for single molecule biophysics: a primer
Author(s) -
Perkins T.T.
Publication year - 2009
Publication title -
laser and photonics reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.778
H-Index - 116
eISSN - 1863-8899
pISSN - 1863-8880
DOI - 10.1002/lpor.200810014
Subject(s) - primer (cosmetics) , trap (plumbing) , optical tweezers , nanotechnology , measure (data warehouse) , physics , engineering physics , computer science , optics , materials science , database , meteorology , thermodynamics
Optical trapping experiments of different complexities are making a significant impact in biology. This review seeks to highlight design choices for scientists entering the field or building new instruments and to discuss making calibrated measurements with optical traps. For specificity, this review focuses on nucleic acid‐based assays, but the discussion reflects the general experimental design considerations of developing a biological assay and an optical trap to measure it.