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Raman spectroscopy and microscopy of individual cells and cellular components
Author(s) -
Chan J.,
Fore S.,
WachsmannHogiu S.,
Huser T.
Publication year - 2008
Publication title -
laser and photonics reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.778
H-Index - 116
eISSN - 1863-8899
pISSN - 1863-8880
DOI - 10.1002/lpor.200810012
Subject(s) - raman spectroscopy , microscopy , raman scattering , chemical imaging , confocal , spectroscopy , nanotechnology , characterization (materials science) , raman microspectroscopy , materials science , fluorescence microscope , fluorescence , biophysics , chemistry , optics , hyperspectral imaging , computer science , biology , physics , artificial intelligence , quantum mechanics
Raman spectroscopy provides the unique opportunity to nondestructively analyze chemical concentrations in individual cells on the submicrometer length scale without the need for optical labels. This enables the rapid assessment of cellular biochemistry inside living cells, and it allows for their continued analysis. Here, we review recent developments in the analysis of single cells, subcellular compartments, and chemical imaging based on Raman spectroscopy. Spontaneous Raman spectroscopy provides for the full spectral assessment of cellular biochemistry, while coherent Raman techniques, such as coherent anti‐Stokes Raman scattering is primarily used as an imaging tool comparable to confocal fluorescence microscopy. These techniques are complemented by surface‐enhanced Raman spectroscopy, which provides higher sensitivity and local specificity, and also extends the techniques to chemical indicators, i.e. pH sensing. We review the strengths and weaknesses of each technique, demonstrate some of their applications and discuss their potential for future research in cell biology and biomedicine.