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Open the Sterivex TM casing: An easy and effective way to improve DNA extraction yields
Author(s) -
Cruaud Perrine,
Vigneron Adrien,
Fradette MarieStéphanie,
Charette Steve J.,
Rodriguez Manuel J.,
Dorea Caetano C.,
Culley Alexander I.
Publication year - 2017
Publication title -
limnology and oceanography: methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.898
H-Index - 72
ISSN - 1541-5856
DOI - 10.1002/lom3.10221
Subject(s) - cartridge , extraction (chemistry) , casing , dna extraction , dna , protocol (science) , filter (signal processing) , chromatography , computer science , biological system , materials science , computational biology , chemistry , biology , engineering , polymerase chain reaction , biochemistry , gene , medicine , alternative medicine , pathology , petroleum engineering , metallurgy , computer vision
We describe an inexpensive, reliable, and easily executed improvement for the extraction of DNA from Sterivex TM filter units, that involves the separation of the Sterivex TM filter from its casing. Our study demonstrates that our modification of the original extraction protocol significantly increased DNA yields, with an average increase of 4.1‐fold more DNA than with the standard approach. A comparison of the diversity after Illumina MiSeq sequencing of bacterial communities extracted with both the standard approach and the proposed one indicated that our modified protocol has no or little impact on the results. This protocol provides a relatively straight forward means to achieve higher yields of DNA from the extraction of Sterivex TM cartridges without altering the community composition and will likely be of interest to a wide range of scientists that use techniques based on the recovery of DNA from filters.