Premium
Comparison of the Effects of Statins on A549 Nonsmall‐Cell Lung Cancer Cell Line Lipids Using Fourier Transform Infrared Spectroscopy: Rosuvastatin Stands Out
Author(s) -
Aksoy Hatice Nurdan,
Ceylan Cagatay
Publication year - 2021
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1002/lipd.12296
Subject(s) - rosuvastatin , pravastatin , fluvastatin , chemistry , statin , rosuvastatin calcium , atorvastatin , a549 cell , simvastatin , pharmacology , hmg coa reductase , fourier transform infrared spectroscopy , cholesterol , biochemistry , cell , medicine , reductase , enzyme , physics , quantum mechanics
Statins are commonly prescribed antilipidemic and anticholesterol class of drugs. In addition to their major role, they have been found to have anticancer effects on in vitro , animal and clinical studies. The aim of this study was to investigate the effects of six different statins (rosuvastatin, pravastatin, simvastatin, lovastatin, fluvastatin, and atorvastatin) on A549 cancer cells lipids by Fourier transform infrared (FTIR) spectroscopy. Proliferation tests were carried out to detect the half‐maximal inhibitory concentrations (IC 50 ) of each statin on A549 cells. The IC 50 values were 50 μM for simvastatin, 150 μM for atorvastatin and pravastatin, and 170 μM for fluvastatin, 200 μM for rosuvastatin and lovastatin on A549 cells. No correlation was found between the antiproliferative effects of the statins and lipid‐lowering effect. The cells were treated with IC 5 , IC 10 , and IC 50 values of each statins concentration and lipid extracts were compared using FTIR spectroscopy. The results indicated that different statins had different effects on the lipid content of A549 cells. The FTIR spectra of the lipid exctracts of statin‐treated A549 cells indicated that the value of hydrocarbon chain length, unsaturation index, oxidative stress level, and phospholipid containing lipids increased except for rosuvastatin‐treated A549 cells. In addition, rosuvastatin significantly lowered cholesterol ester levels. In conclusion, the contrasting effects of rosuvastatin should be further investigated.