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Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase‐1 Gene and a myo ‐Inositol 1‐Phosphate Synthase Knockout Mutation
Author(s) -
ALAmery Maythem,
Fowler Ashley,
Unrine Jason M.,
Armstrong Paul,
Maghirang Elizabeth,
Su Kai,
Melo Julia,
Yuan FengJie,
Shu QingYao,
Hildebrand David
Publication year - 2020
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1002/lipd.12253
Subject(s) - diacylglycerol kinase , soybean meal , soybean oil , biochemistry , biology , phosphate , food science , chemistry , enzyme , raw material , ecology , protein kinase c
Soybean ( Glycine max ) meal is an important protein source. Soybean meal with lower phytate and oligosaccharides improves meal quality. A single recessive mutation in soybean myo‐ inositol 1‐phosphate synthase ( Gm‐lpa ‐TW75‐1) confers a seed phenotype with low phytate and increased inorganic phosphate. The mutant was crossed with high oil lines expressing a diacylglycerol acyltransferase1 (DGAT) gene from Vernonia galamensis (VgD). Gm‐lpa ‐TW75‐1 X VgD, designated GV, has 21%, and 22% oil and 41% and 43% protein from field and greenhouse seed production, respectively. No significant differences were found in mineral concentrations except for Fe which was 229 μg/g dry mass for GV followed by 174.3 for VgD and 162 for Gm‐lpa ‐TW75‐1. Phosphate (Pi) is higher in Gm‐lpa ‐TW75‐1 as expected at 5 mg/g, followed by GV at 1.6 mg/g whereas Jack, VgD, and Taiwan75 have about 0.3 mg/g. The Gm‐lpa ‐TW75‐1 line has the lowest phytate concentration at 1.4 mg/g followed by GV with 1.8 mg/g compared to Taiwan75, VgD, and Jack with 2.5 mg/g. This work describes a high oil and protein soybean line, GV, with increased Pi and lower phytate which will increase the nutritional value for human and animal feed.