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Adipose‐Specific Expression, Developmental and Nutritional Regulation of the Gene‐Encoding Retinol‐Binding Protein 7 in Pigs
Author(s) -
Ahn Jinsoo,
Suh Yeunsu,
Lee Kichoon
Publication year - 2019
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1002/lipd.12170
Subject(s) - adipose tissue , biology , adipogenesis , stromal vascular fraction , gene expression , endocrinology , medicine , regulation of gene expression , clinical chemistry , lipid metabolism , gene , downregulation and upregulation , microbiology and biotechnology , biochemistry
Modulation of expression of adipose tissue‐specific transcripts has been known to regulate adipogenesis and lipid metabolism. Recently, adipose‐specific expression patterns and developmental regulation of the gene‐encoding retinol‐binding protein 7 (RBP7) was identified. However, its expression in adipose tissue of the porcine species has yet to be explored. In this study, adipose tissue‐specific expression of porcine RBP7 was identified and conservation of the fatty acid‐binding domains and evolutionary relationship of the RBP7 gene were verified comparatively across mammalian species. Our in vitro and in vivo analysis of gene expression revealed that RBP7 expression was significantly high in fat cell fraction compared to stromal vascular cells ( p < 0.05) and increased during development ( p < 0.05). The level of RBP7 expression was upregulated during a 24‐h short‐term fasting intervention and restored 6 h after refeeding ( p < 0.05). Taken together, these studies provide insights into the role of RBP7 in adipose tissue of pigs during development and nutritional intervention and pave the way for future studies on the regulation of retinol homeostasis in porcine adipose tissue.