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Exogenously Added Oxyphytosterols Do Not Affect Macrophage‐Mediated Inflammatory Responses
Author(s) -
Oligschlaeger Yvonne,
Houben Tom,
Jeurissen Mike L. J.,
Bitorina Albert V.,
Konings Maurice,
Baumgartner Sabine,
Plat Jogchum,
ShiriSverdlov Ronit
Publication year - 2018
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1002/lipd.12044
Subject(s) - proinflammatory cytokine , campesterol , macrophage , scavenger receptor , cholesterol , chemistry , clinical chemistry , endocrinology , receptor , medicine , phytosterol , inflammation , sterol , biochemistry , lipoprotein , in vitro , microbiology and biotechnology , biology
Although phytosterols, plant‐derived sterol‐like components, are well known for their cholesterol‐lowering properties, their atherogenic potential is still under debate. Although they are known to share structural similarities with cholesterol, it is unclear whether their oxidized forms (oxyphytosterols) have the capacity to mediate proinflammatory responses in macrophages. In the present study, bone marrow‐derived macrophages were treated with oxidized low‐density lipoproteins, oxyphytosterols (7keto‐sito/campesterol [7keto‐sit/camp] or 7‐beta‐hydroxy‐sito/campesterol [7βOH‐sit/camp]), nonoxidized phytosterol (β‐sitosterol), or carrier‐control (cyclodextrin) in a dose‐ and time‐dependent manner. Inflammatory cytokine release, activity, and the corresponding mRNA expression levels were analyzed. 7βOH‐sit/camp, rather than 7keto‐sit/camp, induced a modest proinflammatory response in wild‐type cells derived from C57Bl/6 mice. The observed mild inflammatory effects are independent of the low‐density lipoprotein receptor and Cluster of differentiation 36/Scavenger receptor‐a. These data suggest that exogenously added oxyphytosterols do not affect macrophage‐mediated inflammatory responses, at least in vitro.