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Adapted MS/MS ALL Shotgun Lipidomics Approach for Analysis of Cardiolipin Molecular Species
Author(s) -
Gao Fei,
McDaniel Justice,
Chen Emily Y.,
Rockwell Hannah E.,
Nguyen Cindy,
Lynes Matthew D.,
Tseng YuHua,
Sarangarajan Rangaprasad,
Narain Niven R.,
Kiebish Michael A.
Publication year - 2018
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1002/lipd.12004
Subject(s) - lipidomics , cardiolipin , mass spectrometry , cardiolipins , electrospray ionization , chemistry , tandem mass spectrometry , shotgun , chromatography , biology , phospholipid , biochemistry , membrane , gene
Cardiolipin (Ptd 2 Gro) is a complex, doubly charged phospholipid located in the inner mitochondrial membrane where it plays an essential role in regulating bioenergetics. Abnormalities in Ptd 2 Gro content or composition have been associated with mitochondrial dysfunction in a variety of disease states. Here, we report the development of an adapted high‐resolution data‐independent acquisition (DIA) MS/MS ALL shotgun lipidomic method to enhance the accuracy and reproducibility of Ptd 2 Gro molecular species quantitation from biological samples. Utilizing the doubly charged molecular ions and the isotopic pattern with negative mode electrospray ionization mass spectrometry (ESI‐MS) using an adapted MS/MS ALL approach, we profiled more than 150 individual Ptd 2 Gro species, including monolysocardiolipin (MLPtd 2 Gro). The method described in this study demonstrated high reproducibility, sensitivity, and throughput with a wide dynamic range. This high‐resolution MS/MS ALL shotgun lipidomics approach could be extended to screening aberrations of Ptd 2 Gro metabolism involved in mitochondrial dysfunction in various pathological conditions and diseases.