Open AccessA bis(pyrazolyl)methane derivative against clinical Staphylococcus aureus strains isolated from otitis externa
Author(s) -
Ocaña Ana V.,
AguileraCorrea John J.,
DomínguezJurado Elena,
PérezMartínez Francisco C.,
PérezTanoira Ramón,
LópezCarretero Yaiza,
MasiáMondejar Jesús,
CastroOsma José Antonio,
Esteban Jaime,
AlonsoMoreno Carlos,
MolinaAlarcón Milagros,
Seguí Pedro
Publication year - 2022
Publication title -
laryngoscope investigative otolaryngology
Language(s) - English
Resource type - Journals
ISSN - 2378-8038
DOI - 10.1002/lio2.722
Subject(s) - staphylococcus aureus , minimum bactericidal concentration , microbiology and biotechnology , morganella morganii , minimum inhibitory concentration , enterobacter aerogenes , pseudomonas aeruginosa , antibiotics , klebsiella pneumoniae , biofilm , otitis , chemistry , bacteria , biology , medicine , escherichia coli , surgery , biochemistry , genetics , gene
Abstract Objective The purpose of this study was to evaluate the in vitro antibacterial effects of a p‐ Cymene‐based bis(pyrazolyl)methane derivative (SC‐19) to advance in developing alternative therapeutic compounds to fight against bacterial isolates from patients with otitis externa (OE). Methods Eighteen swab specimens were collected from patients aged over 18 years diagnosed with OE within at least 7 days of symptom onset, contaminated by only one bacterium type: Pseudomonas aeruginosa ( n = 5); Staphylococcus aureus ( n = 8); Klebsiella aerogenes ( n = 2); Serratia marcescens ( n = 1); Morganella morganii ( n = 2). To appraise antibacterial activity, minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), and minimum biofilm eradication concentration (MBEC) assays were run at different SC‐19 concentrations. Results When using SC‐19, S. aureus strains showed less bacterial growth, but no bactericidal effect was observed. The MIC and MBC of SC‐19 were 62.5 and 2000 μg/ml against S. aureus and were >2000 μg/ml against the other isolates obtained from OE, respectively. In addition, the MBICs and MBECs of SC‐19 against S. aureus were 125 and >2000 μg/ml, respectively. Conclusion Nowadays the acquired antibiotic resistance phenomenon has stimulated research into novel and more efficient therapeutic agents. Hence, we report that, helped by the structural diversity fostered herein by a range of bis(pyrazolyl)methane derivatives, SC‐19 can be a promising alternative therapeutic option for treating OE caused by S. aureus given the observed effects on both planktonic state and biofilm. Level of Evidence IV