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Regulatory Control of Energy Drinks Using 1 H NMR Spectroscopy
Author(s) -
Wegert K.,
Monakhova Y. B.,
Kuballa T.,
Reusch H.,
Winkler G.,
Lachenmeier D. W.
Publication year - 2012
Publication title -
lebensmittelchemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3811
pISSN - 0937-1478
DOI - 10.1002/lemi.201290113
Subject(s) - chemistry , nuclear magnetic resonance spectroscopy , context (archaeology) , spectroscopy , taurine , caffeine , detection limit , phosphate buffered saline , analytical chemistry (journal) , calibration curve , chromatography , stereochemistry , biochemistry , physics , medicine , paleontology , amino acid , quantum mechanics , biology , endocrinology
400 MHz 1 H nuclear magnetic resonance (NMR) spectroscopy was used in the context of food surveillance to control the regulatory limits of selected constituents of energy drinks. The preparation of the samples required only degassing, addition of 0.1 % of TSP in D 2 O for locking and referencing and phosphate buffer (pH 4.0) for chemical shifts adjustment. The quantification of caffeine, glucuronolactone, taurine and inositol was simultaneously possible using external calibration curves and applying TSP as internal standard. The applicability of the method was proven by analysis of 73 samples of energy drinks. The average caffeine concentration was 0.30 g/L, which is below the maximum limit of 0.32 g/L.