Open AccessCommon bean ( Phaseolus vulgaris L.) with increased cysteine and methionine concentration
Author(s) -
ViscarraTorrico Rosa Cecilia,
Pajak Aga,
Garzón Alvaro Soler,
Zhang BaiLing,
Pandurangan Sudhakar,
Diapari Marwan,
Song Qijian,
Conner Robert L.,
House James D.,
Miklas Phillip N.,
Hou Anfu,
Marsolais Frédéric
Publication year - 2021
Publication title -
legume science
Language(s) - English
Resource type - Journals
ISSN - 2639-6181
DOI - 10.1002/leg3.103
Subject(s) - methionine , phaseolus , biology , cysteine , amino acid , protein quality , recombinant dna , quantitative trait locus , genotype , population , inbred strain , single nucleotide polymorphism , biochemistry , horticulture , gene , enzyme , demography , sociology
In common bean ( Phaseolus vulgaris L.), seed storage protein deficiency is associated with increased total cysteine and methionine concentration. The goal of this study was to generate germplasm lines that combine this characteristic with adaptation to short season conditions in Manitoba, Canada. A recombinant inbred line population was developed by crossing the storage protein deficient genotype, SMARC1N‐PN1 with the cultivar Morden003. Two lines, 2‐37 and 3‐84, with a stable protein profile over 2 years at two locations were identified. Like SMARC1N‐PN1, both lines had a significantly higher cysteine concentration than Morden003, by approximately 35%. Methionine levels were elevated by approximately 15%, while tryptophan levels were also increased by approximately 30%. Line 2‐37 had a significant increase in protein quality, as measured by in vitro protein digestibility corrected amino acid score, by approximately 40%, as compared with Morden003. The increased protein quality for this line is attributable to higher levels of total cysteine and methionine, while having an overall reduction in crude protein concentration. Line 2‐37 had a similar seed yield as SMARC1N‐PN1, with a maturity comparable to Morden003. The results of high‐density single nucleotide polymorphism (SNP) genotyping and quantitative trait locus analysis of recombinant inbred lines indicated that variation in cysteine concentration was determined by the phaseolin locus, while variation in methionine concentration was determined by both the phaseolin and lectin loci. SNP markers that track the introgression of phaseolin and lectin deficiency into the Morden003 background were identified and validated.