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LncRNA miR143HG suppresses miR‐21 through methylation to inhibit cell invasion and migration
Author(s) -
Xun Wu,
Cen Wei,
Dahai Yu,
Huaqing Wei,
Jiping Su,
Mengzhu Guo,
Ning Meng
Publication year - 2020
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.28474
Subject(s) - methylation , cell migration , cell , cancer research , transfection , dna methylation , microbiology and biotechnology , biology , cell growth , real time polymerase chain reaction , microrna , gentamicin protection assay , western blot , cell culture , gene expression , gene , genetics
Objective This study aimed to investigate the role of lncRNA miR143HG in laryngeal squamous cell carcinoma (LSCC). Methods Quantitative polymerase chain reaction (PCR) and paired t test were used to measure and compare expression levels of miR143HG and miR‐21 in LSCC and nontumor tissues. To analyze the interactions between miR143HG and miR‐21, UM‐SCC‐17A cells were transfected miR143HG expression vector or miR‐21 mimic. The effects of miR143HG and miR‐21 overexpression on UM‐SCC‐17A cell invasion and migration were analyzed by transwell assays. Results We found that miR143HG was downregulated in LSCC and inversely correlated with miR‐21. In LSCC cells, miR143HG overexpression led to the downregulated expression of miR‐21, whereas miR‐21 overexpression failed to affect miR143HG. Methylation‐specific PCR results showed that miR143HG overexpression led to increased methylation of miR‐21. Low expression levels of miR143HG were correlated with poor survival. Overexpression of miR143HG led to decreased, whereas miR‐21 overexpression resulted in increased rate of LSCC cell migration and invasion. In addition, miR‐21 overexpression led to reduced effects of miR143HG on cell invasion and migration. Conclusion Therefore, miR143HG suppresses miR‐21 via methylation to regulate cell behaviors in LSCC. Level of Evidence NA Laryngoscope , 130:E640–E645, 2020