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Does Salivary Function Decrease in Proportion to Radioiodine Dose?
Author(s) -
Kim Ji Won,
Kim Jeong Mi,
Choi Mi Eun,
Kim SeokKi,
Kim YoungMo,
Choi JeongSeok
Publication year - 2020
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.28342
Subject(s) - salivary gland , saliva , medicine , endocrinology , immunohistochemistry , apoptosis , oxidative stress , submandibular gland , atrophy , chemistry , biochemistry
Objectives This study was conducted to investigate the dose‐response characteristics of radioiodine on salivary glands and to investigate the mechanism responsible for radioiodine‐induced salivary glands toxicity. Methods Twenty‐four mice were divided into six groups: 0, 0.05, 0.10, 0.20, 0.40, and 0.80 mCi/20 g mouse, administered orally. Mortalities were noted 12 months after radioiodine administration. Body weights, gland weights, salivary lag times, flow rates, and changes in 99m Tc pertechnetate were recorded. Histopathological changes and mRNA expressions were also evaluated, and immunohistochemical analysis and apoptotic assays were performed. Results Survival rates, body weights, gland weights, and flow rates decreased, and lag times increased on increasing radioiodine dose. Animals administered radioiodine showed acinar atrophy, striated duct dilations, and lymphocytic infiltration in glands and irregular destruction of epithelial surfaces of tongue. The uptake and excretion of 99m Tc pertechnetate were impaired by radioiodine. Immunohistochemical analysis showed that numbers of salivary epithelial, myoepithelial, and endothelial cells decreased and that numbers of ductal cells increased with radioiodine dose. Oxidative stress biomarker levels increased; reactive oxygen species scavenger levels decreased; and numbers of apoptotic cells increased in animals exposed to higher radioiodine doses. Conclusion These dose‐related, long‐term effects on salivary gland should be taken into account when determining radioiodine doses. Level of Evidence NA Laryngoscope , 130:2173–2178, 2020