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microRNAs in sudden hearing loss
Author(s) -
Nunez Desmond A.,
Wijesinghe Printha,
Nabi Shahin,
Yeh David,
Garnis Cathie
Publication year - 2020
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.28327
Subject(s) - microrna , medicine , prospective cohort study , protein kinase b , messenger rna , cohort , kinase , biology , mapk/erk pathway , signal transduction , bioinformatics , gene , genetics
Objectives To compare the circulating microRNA (miRNA) expression profiles between sudden sensory neural hearing loss (SSNHL) patients and age‐matched normal hearing controls. Study Design Prospective cohort multi‐center study. Methods Patients presenting within 28 days of onset of SSNHL were prospectively recruited along with contemporaneous age‐matched controls. Pooled sera of four patient (n = 09, mean age = 53.0 years; 07, 55.0; 10, 52.9; 10, 51.6) and two control (09, 51.2 and 03, 50.0) groups were assessed using a TaqMan Low Density Array. The patients’ sera were also divided into two pools, untreated (04, 57.7) and treated (32, 52.6) for additional analysis. miRNA expression level was derived from cycle threshold (Ct) values normalized to a global mean. Inter‐group mean Ct differences with fold changes ≥2.0 and ≤0.5 at P < .05 were considered significant. Bioinformatic databases were used to identify putative target mRNAs or validated genes and their functional annotations. Results Thirty‐six SSNHL patients (mean age 53.0 ± standard deviation (SD) 15.2 years) and 12 controls (50.9 ± 11.9) were studied. Eight miRNAs hsa‐miR‐590‐5p/ ‐186‐5p/ ‐195‐5p/ ‐140‐3p/ ‐128‐3p/ ‐132‐3p/ ‐375‐3p, and ‐30a‐3p were identified as significantly differentially expressed in SSNHL patients. Most of these miRNAs were abundantly identified in the nervous system and the putative target messenger RNAs (mRNAs) were enriched in signaling pathways such as phosphatidyl inositol 3 kinase/protein Kinase B (PI3K/Akt), Ras and mitogen‐activated protein kinase (MAPK). Conclusion These findings suggest the possible cellular signaling pathways that underlie the disruption of auditory signal transmission in SSNHL. Level of Evidence 2 Laryngoscope, 130:E416–E422, 2020