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A 3D‐printed polycaprolactone/β‐tricalcium phosphate mandibular prosthesis: A pilot animal study
Author(s) -
Park Jae Hong,
Jung Soo Yeon,
Lee ChiKyou,
Ban Myung Jin,
Lee Sang Jin,
Kim Ha Yeong,
Oh Hyun Ju,
Kim Byeong Kook,
Park Hae Sang,
Jang SiHyong,
Kim Han Su
Publication year - 2020
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.27908
Subject(s) - scaffold , mesenchymal stem cell , chemistry , biomedical engineering , mandible (arthropod mouthpart) , regeneration (biology) , polycaprolactone , dentistry , bone tissue , anatomy , medicine , pathology , biology , microbiology and biotechnology , botany , genus , organic chemistry , polymer
Objective In this study, we assessed the effectiveness of a tonsil‐derived mesenchymal stem cell (TMSC)‐transplanted polycaprolactone/beta‐tricalcium phosphate prosthesis (specifically designed for easier fixing and grafting with a single scaffold) on rabbit mandible osteogenesis. Methods The mandibles of 18 rabbits were exposed, and 10 × 8‐mm bone defects were made. Two rabbits did not receive implants; four were reconstructed with the scaffold control (SC) (SC group); four were reconstructed with scaffolds soaked in peripheral blood (PB) (PB group); four were reconstructed with TMSC‐transplanted scaffolds (TMSC group); and four were reconstructed with differentiated osteocyte‐transplanted scaffolds (DOC) (DOC group). Each rabbit was sacrificed 12 weeks after surgery, and the area of new bone formation was investigated by mechanical testing, histology, and micro‐computed tomography. Results More extended and denser new bone masses were observed in the TMSC and DOC groups, although fibrosis and vascular formation levels were similar in all groups, suggesting that the dual‐structured scaffold alone provides a good environment for bone attachment and regeneration. The bone volumes of representative scaffolds from the SC, PB, TMSC, and DOC groups were 43.12, 48.35, 53.10, and 57.44% of the total volumes, respectively. Conclusion The design of the scaffold resulted in effective osteogenesis, and TMSCs showed osteogenic potency, indicating that their combination could enable effective bone regeneration. Level of Evidence NA Laryngoscope , 130:358–366, 2020