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Alterations in macrophage polarization in injured murine vocal folds
Author(s) -
Kaba Shinji,
Nakamura Ryosuke,
Yamashita Masaru,
Katsuno Tatsuya,
Suzuki Ryo,
Tateya Ichiro,
Kishimoto Yo,
Omori Koichi
Publication year - 2019
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.27523
Subject(s) - macrophage polarization , lamina propria , macrophage , m2 macrophage , vocal folds , nitric oxide synthase , population , immunohistochemistry , phenotype , biology , wound healing , pathology , nitric oxide , immunology , andrology , medicine , anatomy , epithelium , endocrinology , in vitro , gene , biochemistry , larynx , environmental health
Objectives Macrophages are prominent inflammatory cells in wounds, and their phenotypes are altered during wound healing. They are reported to contribute to not only inflammatory responses but also tissue remodeling. However, few studies in vocal fold biology have focused on the function of macrophages. The purpose of this study was to investigate macrophage polarization and distribution in injured murine vocal folds. Study Design Animal experiments with controls. Method Unilateral vocal fold stripping was performed on C57BL/6 mice, and larynges were harvested 1, 3, 5, 7, and 14 days postinjury. Immunohistochemical analysis of the vocal fold lamina propria was performed to detect the expression of classically activated (M1) and alternatively activated (M2) macrophage markers (inducible nitric oxide synthase [iNOS] and CD206, respectively) in F4/80 + macrophages. Results The proportion of F4/80 + iNOS + cells out of all F4/80 + cells tended to increase from day 1. F4/80 + iNOS + cell percentage tended to be high at days 1 through 7 and declined to close to a normal level by day 14. F4/80 + CD206 + cell percentage tended to decrease at day 1 and then to increase the rest of the time. In the normal vocal fold, the majority of F4/80 + macrophages were only positive for CD206. F4/80 + iNOS + CD206 + cells were observed at days 1 through 7. Conclusion The main population of injured sites gradually shifted from M1 to M2 marker‐positive macrophages in murine vocal folds. However, coexistence of M1 and M2 markers in the same macrophages was observed. Our results suggest that macrophage phenotypes are regulated by complex tissue‐derived signals and exhibit dynamic changes during wound healing. Level of Evidence NA Laryngoscope , 129:E135–E142, 2019