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Oral sampling methods are associated with differences in immune marker concentrations
Author(s) -
Fakhry Carole,
Qeadan Fares,
Gilman Robert H.,
Yori Pablo,
Kosek Margaret,
Patterson Nicole,
Eisele David W.,
Gourin Christine G.,
Chitguppi Chandala,
Marks Morgan,
Gravitt Patti
Publication year - 2018
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.27002
Subject(s) - saliva , immune system , multiplex , immunology , biology , medicine , bioinformatics
Objective To determine whether the concentration and distribution of immune markers in paired oral samples were similar. Study Type Clinical research. Study Design Cross‐sectional study. Methods Paired saliva and oral secretions (OS) samples were collected. The concentration of immune markers was estimated using Luminex multiplex assay (Thermo Fisher Scientific, Waltham, MA). For each sample, the concentration of respective immune markers was normalized to total protein present and log‐transformed. Median concentrations of immune markers were compared between both types of samples. Intermarker correlation in each sampling method and across sampling methods was evaluated. Results There were 90 study participants. Concentrations of immune markers in saliva samples were significantly different from concentrations in OS samples. Oral secretions samples showed higher concentrations of immunoregulatory markers, whereas the saliva samples contained proinflammatory markers in higher concentration. Conclusion The immune marker profile in saliva samples is distinct from the immune marker profile in paired OS samples. Level of Evidence 2b. Laryngoscope , 128:E214–E221, 2018