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Use of autologous adipose‐derived mesenchymal stem cells for creation of laryngeal cartilage
Author(s) -
Zhang Hongji,
VoytikHarbin Sherry,
Brookes Sarah,
Zhang Lujuan,
Wallace Joseph,
Parker Noah,
Halum Stacey
Publication year - 2018
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.26980
Subject(s) - cartilage , chondrogenesis , mesenchymal stem cell , tissue engineering , chondrocyte , adipose tissue , stem cell transplantation for articular cartilage repair , staining , pathology , extracellular matrix , matrix (chemical analysis) , microbiology and biotechnology , biomedical engineering , anatomy , chemistry , in vitro , biology , medicine , adult stem cell , biochemistry , endothelial stem cell , chromatography
Objectives/Hypothesis Adipose‐derived mesenchymal stem cells (ASCs) are an exciting potential cell source for tissue engineering because cells can be derived from the simple excision of autologous fat. This study introduces a novel approach for tissue‐engineering cartilage from ASCs and a customized collagen oligomer solution, and demonstrates that the resultant cartilage can be used for laryngeal cartilage reconstruction in an animal model. Study Design Basic science experimental design. Methods ASCs were isolated from F344 rats, seeded in a customized collagen matrix, and cultured in chondrogenic differentiation medium for 1, 2, and 4 weeks until demonstrating cartilage‐like characteristics in vitro. Large laryngeal cartilage defects were created in the F344 rat model, with the engineered cartilage used to replace the cartilage defects, and the rats followed for 1 to 3 months. Staining examined cellular morphology and cartilage‐specific features. Results In vitro histological staining revealed rounded chondrocyte‐appearing cells evenly residing throughout the customized collagen scaffold, with positive staining for cartilage‐specific markers. The cartilage was used to successfully repair large cartilaginous defects in the rat model, with excellent functional results. Conclusions This study is the first study to demonstrate, in an animal model, that ASCs cultured in a unique form of collagen oligomer can create functional cartilage‐like grafts that can be successfully used for partial laryngeal cartilage replacement. Level of Evidence NA. Laryngoscope , 128:E123–E129, 2018