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Endoscopic management of tissue‐engineered tracheal graft stenosis in an ovine model
Author(s) -
Pepper Victoria K.,
Onwuka Ekene A.,
Best Cameron A.,
King Nakesha,
Heuer Eric,
Johnson Jed,
Breuer Christopher K.,
Grischkan Jonathan M.,
Chiang Tendy
Publication year - 2017
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.26504
Subject(s) - medicine , fluoroscopy , bronchoscopy , stenosis , stent , lumen (anatomy) , tracheal stenosis , dilation (metric space) , radiology , surgery , mathematics , combinatorics
Objective To evaluate the safety and efficacy of bronchoscopic interventions in the management of tissue‐engineered tracheal graft (TETG) stenosis. Study Design Animal research study. Methods TETGs were constructed with seeded autologous bone marrow‐derived mononuclear cells on a bioartificial graft. Eight sheep underwent tracheal resection and orthotopic implantation of this construct. Animals were monitored by bronchoscopy and fluoroscopy at 3 weeks, 6 weeks, 3 months, and 4 months. Bronchoscopic interventions, including dilation and stenting, were performed to manage graft stenosis. Postdilation measurements were obtained endoscopically and fluoroscopically. Results Seven dilations were performed in six animals. At the point of maximal stenosis, the lumen measured 44.6 ± 8.4 mm 2 predilation and 50.7 ± 14.1 postdilation by bronchoscopy ( P = 0.3517). By fluoroscopic imaging, the airway was 55.9 ± 12.9 mm 2 predilation and 65.9 ± 22.4 mm 2 postdilation ( P = 0.1303). Stents were placed 17 times in six animals. Pre‐ and poststenting lumen sizes were 62.8 ± 38.8 mm 2 and 80.1 ± 54.5 mm 2 by bronchoscopy ( P = 0.6169) and 77.1 ± 38.9 mm 2 and 104 ± 60.7 mm 2 by fluoroscopy ( P = 0.0825). Mortality after intervention was 67% with dilation and 0% with stenting ( P = 0.0004). The average days between bronchoscopy were 8 ± 2 for the dilation group and 26 ± 17 in the stenting group ( P = 0.05). One hundred percent of dilations and 29% of stent placements required urgent follow‐up bronchoscopy ( P = 0.05). Conclusion Dilation has limited efficacy for managing TETG stenosis, whereas stenting has a more lasting clinical effect. Level of Evidence NA. Laryngoscope , 127:2219–2224, 2017